Sunghoon Kwon, Seoul National University

Title: Translation from Lab to hospital: Rapid Antibiotic Susceptibility Test & DNA Laser Printer
Session: Tuesday, 6 October, 10:15

Abstract

It is always exciting to see how academic inventions translated into commercial impact. I will present two recent academic results of Biophotonics and Nanoengineering laboratory at Seoul National University that had successful translation to personalized in-vitro diagnostic products. I will share details of technology and commercialization of rapid antibiotic susceptibility test using microfluidics [1] and ultra high throughput DNA synthesis using laser sniping [2].
Rapid Antibiotic Susceptibility Test
A rapid antibiotic susceptibility test (RAST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs are not suitable for urgent cases of bacterial infection and antibiotic resistance due to their relatively long test time. I will present a fast AST method named single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. With SCMA, AST is finished only in 3 hours satisfying FDA guidelines. I will discuss application of SCMA to fast diagnostics of multi-drug resistant tuberculosis.
DNA Laser Printer
Writing DNA serves significant purposes in the fields of synthetic biology, functional genomics, and bioengineering. The megaclones on next generation sequencing (NGS) platform have potential to be a rich and cost-effective source of sequence-verified DNAs as a precursor for DNA writing. I will present an enabling technology named, 'Sniper Cloning', that enables the precise mapping of target clone features on NGS platform and noncontact rapid retrieval of targets for full utilization of megaclones. By merging the three cutting-edge technologies of NGS, DNA microarray and our pulse laser retrieval system, we have lowered cost for DNA synthesis two orders of magnitude. I will present synthesis of shRNA library and target capture panel for NGS and their clinical utility.

[1] "A rapid antimicrobial susceptibility test based on single-cell morphological analysis", Science Translational Medicine, Volume 6, Issue 267.
[2] "Sniper Cloning: A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from NGS platform", Nature Communications, volume 6, 2015

 

 

Bio

Sunghoon Kwon is an associate professor in Electrical Engineering at Seoul National University in Korea. Before he joined SNU, he gained multidisciplinary academic training: BS in Electrical Engineering (at SNU 1998), MS in Medical Engineering (at SNU hospital, 2000), Ph.D in Bioengineering (joint degree at UCSF/UCBerkeley 2004) and Postdoc in nanofabrication facility for nanoscience (at Molecular Foundry, Lawrence Berkeley National Laboratory, 2004-2006). With emphasis on creativity from multidisciplinary fusion, his research group, Biophonics and Nanoengineering Laboratory (BiNEL), is pursuing multidisciplinary research in BioMEMS, Photonics, and Nanofabrication to deliver technological innovations via increasing functional throughput of devices and systems at all scale. Current research interests include guided-self assembly, high throughput drug screening, and artificial structural colors, and high throughput gene synthesis technology. He received the Young Scientist Award from the President of Korea (2011), Creative Advanced Researcher Professorship (2012), Excellent Research Award from Seoul National University (2010), and Doyoen Creative Research Award from International Semiconductor Research Center (2009). He is one of eight SNU Distinguished University Professor in Seoul National University. He founded QuantaMatrix and Celemics that commercializes personalized diagnostic solutions using micro-nano technologies.

 

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