Gerrit Sitters, Lumicks/VU University


Force-spectroscopy has become an indispensable tool to unravel the structural and mechanochemical properties of biomolecules. Acoustic Force Spectroscopy (AFS) is a new acoustic manipulation method, which consists of a resonator integrated into a micro-fabricated fluidic chip. An acoustical pressure gradient is created homogeneously throughout the sample enabling to exert forces on DNA-tethered microspheres. By changing the amplitude of the driving voltage the pressure gradient can be altered, allowing sensitive control of the force applied to the DNA molecules. This approach allows exerting acoustic forces from sub-pN to hundreds of pN applied to thousands of biomolecules in parallel, with sub-millisecond response time and inherent stability. Here we present our efforts in validating this technology by developing a stand-alone commercial-grade instrument that allows ease of operation.


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