Douwe Kamsma, VU Amsterdam

Title: Acoutic Force Spectroscopy (AFS)
Session: Monday 5 October, 15:00


Single-molecule force spectroscopy has become an indispensable tool to unravel the structural and mechano-chemical properties of biomolecules. Here we extend the force spectroscopy toolbox with an acoustic manipulation device that can exert forces on tethered microspheres.
AFS is a Lab-on-a-chip device consisting of a flow cell with a piezo element glued on top. While applying an alternating voltage to the piezo element, forces from subpiconewtons to hundreds of piconewtons can be reached. As a proof of concept we performed force-extension measurements on DNA and RecA-coated DNA. Also, we mapped the energy landscape of the DIG/anti-DIG antibody-antigen bond, by measuring the rupture force of >2000 molecules in parallel, over 6 orders of magnitude of force loading rates within 2 days of experimentation.
These aspects will help to spread single-molecule methods from the realm of fundamental research in specialized laboratories towards more wide-spread applications in for example molecular biology and medical diagnostics.





 to be announced


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